Benutzeranleitung / Produktwartung MZ16 F des Produzenten Leica
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Leica MZ16 F User manual.
Leica MZ16 F – User manual 2 2 Leica MZ16 F – User manual Dear User , W e thank you for your faith in our product, and hope it brings you both enjoyment and success. With the new fully apochromatic Leica MZ16 F from Leica Microsystems, you have purchased the best-performing, manual fluorescence stereomi- croscope on the market.
3 Leica MZ16 F – User manual Operating controls 1 Zoom magnification changer 2 Coarse/fine focusing drive 3 Double iris diaphragm 4 Rapid filter changer for four selectable filter sets 5 Filter hold.
Page 4. Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 4.1 Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18 4.1.1 Fluorescence technology . . . . . . . . . . .18 4.1.2 Functional principle . . . . . . . .
Leica MZ16 F – User manual 5 Leica MZ16 F – Safety concept 5 1. Safety concept 1.1 The user manual Included with your Leica MZ16 F fluorescence stereomicroscope is an interactive CD-ROM with all pertinent user instructions in German, English, French, Spanish, Italian, Portuguese, Dutch, Dan- ish, Swedish, Finnish and Greek.
6 Leica MZ16 F – Safety concept 1.1.2 Symbols used Warning of a danger This symbol indicates especially important infor- mation that, if not observed, – can cause hazards to personnel, – can lead to functional disturbances and dam- aged instruments.
Leica MZ16 F – User manual 7 Leica MZ16 F – Safety concept Permitted uses With the Leica MZ16 F fluorescence stereomicro- scope, entire fluorescent specimens can be examined three-dimensionally and unprepared, manipulated, sorted and documented.
8 Leica MZ16 F – Safety concept Repairs and servicing – Only Leica Microsystems-trained service tech- nicians, or technical specialists assigned by the person in charge of the instrument, are permitted to carry out repairs. – Only original Leica Microsystems spare parts may be used.
Leica MZ16 F – Safety concept 9 1.3 Light source: safety instructions Precautions by the manufacturer – UV protection screen in front of the specimen plane prevents the user from looking directly into the UV rays.
10 Leica MZ16 F – Overview of the instrument 2. Overview of the instrument 1 Stand (transmitted or incident light stand with corresponding illumination).
Leica MZ16 F – Overview of the instrument 11 7 Filter sets: the scope of delivery includes three simple filter holders (which have only two openings for the observation beam paths) for empty filter positions and transmitted or inci- dent light observation without fluorescence.
12 Leica MZ16 F – Assembly 3. Assembly Basic components such as the stand, column, microscope carrier , binocular tubes and optical accessories are, thanks to their modular design, identical for all Leica M series stereomicro- scopes. Therefore, for assembly instructions, please refer to the User Manual for Leica M stereomicroscopes, M2-105-0.
Leica MZ16 F – Assembly 13 10 445 631 10 445 367 HL 10 445 363 10 445 387 10 445 822 10 446 229 Filter Sets 10 447 220 10 447 221 10 447 217 10 447 222 10 447 218 10 447 223 10 447 219 10 447 224 10.
14 Leica MZ16 F – Assembly 3.2 Assembly of stereomicroscope with stand Do not yet connect the peripherals to the power supply! Focusing drive ➔ stand base 왘 Fasten the manual or motorized focusing drive (4.2) to the column according to User Manual M2-105-0 (manual) or M2-267-1 (motorized) on the base (4.
Leica MZ16 F – Assembly 15 3.3 Additional components 왘 Fasten the other components and other individ- ual accessories according to User Manual M2-105-0: – Insert the binocular tube (2.5) or the video/ phototube into the dovetail ring on the FLUOIII ® filter system and fasten it using a clamping screw .
16 Leica MZ16 F – User manual Leica MZ16 F – Assembly 3.5 UV protection screen ➔ microscope carrier 왘 Fasten the UV protection screen (5.1) using a hexagon-head screw (5.3) to the left or right side of the microscope carrier . T ool: Allen key .
Leica MZ16 F – User manual 17 Leica MZ16 F – Assembly 1 2 3.6.2 Simple filter holders Three simple filter holders are included in the scope of delivery of the Leica MZ16 F . These filter holders have two empty openings for the observa- tion beam paths.
18 Leica MZ16 F – Operation 4. Operation 4.1 Description 4.1.1 Fluorescence technology Fluorescent substances light up (fluoresce) when irradiated with short-wave excitation light.
Leica MZ16 F – Functions 19 5.1.1 Observation without fluorescence The FLUOIII ® filter system includes a UV shutter that can be used to close the illumination beam path. This feature protects the mercury lamp, which should not be switched on and off too fre- quently .
20 Leica MZ16 F – Functions 5.3 Rapid filter changer The rapid filter changer must be equipped with four filter holders at all times. If you are using fewer than four filter hold- ers with fluorescence filters, always insert the included simple filter holders into the available positions.
Leica MZ16 F – Work flow 21 6. W ork flow 왘 Switch on the supply unit (page 15). W ait for 2 to 3 minutes. 왘 Close the illumination beam path using the switch (10.1). 왘 Correctly set the binocular tube, eyepieces and working distance in accordance with the User Manual M2-105-0.
22 Leica MZ16 F – Appendix 7. Appendix 7.1 Filter sets Filter sets Excitation filter Barrier filter Designation GFP 425/60 nm (395–455 nm) 480 LP GFP1 GFP Plus 480/40 nm (460–500 nm) 510 LP GFP2.
Leica MZ16 F – Appendix 23 7.2 T echnical data Optics carrier Leica MZ16 F Microscope type stereo fluorescence microscope with apochromatic optics system, patented T ripleBeam ® third beam path, pa.
24 Leica MZ16 F – Appendix Leica MZ16 F Optical Data Zoom 16:1 manual, apochromatic Selectable zoom positions Click-stops at 0.71 | 1 | 1.6 | 2 | 2.5 | 3.2 | 4 | 5 | 6.3 | 8 | 10 | 11.5 for repetitive tasks Performance with standard optics (1 × objective/10 × eyepieces) – Zoom range 7.
Leica MZ16 F – Appendix 25 Stands, illuminators Leica MZ16 F T ransmitted light stand HL RC™ high-performance base with innovative contrast meth- ods, stands for bright field and bright/dark field.
26 Leica MZ16 F – Appendix Leica MZ16 F T echnical Data Weights – Leica MZ16 F optics carrier – Lamp housing 2.5 kg – Filter set 20 g – Planapochromatic objective 1 × 0.74 kg – Focusing drive, coarse/fine, with column 1.15 kg – Microscope carrier 0.
Leica MZ16 F – User manual 27 Leica MZ16 F – Appendix 27 7.3 Optical data Field of view diameter (mm) Eyepieces Working distances in mm Objectives 0.71 28.4 8.4 14.2 16.9 17.9 13.4 22.7 10.5 45.4 5.3 56.8 4.2 0.8 32 7.5 16 15 20.5 11.7 25.6 9.4 51.
7.4 Dimensions 28 Leica MZ16 F – Appendix Dimensions in mm.
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