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Leica M165 FC Leica M205 F A User Manual.
Leica M-series User Manual 2 General Instructions Safety concept Before using your microscope for the first time, please read the "Safety concept" br ochure included with your instrument. It contains addi - tional information about handling and care .
Leica M-series User Manual 3 Impor tant Safety Notes User Manual The individual modules of the Leica M stereo - microscopy series include an interactive CD-ROM with all relevant user manuals in 20 other languages. Keep it in a safe place, and readily accessible to the user .
Leica M-series User Manual 4 Symbols U sed W arning of a danger • This symbol indicates especially impor - tant information that must be read and complied with.
Leica M-series User Manual 5 Safety Instruc tions Description The individual modules fulfill the highest requirements for obser vation and documenta - tion of Leica ster eomicroscopes of the M series .
Leica M-series User Manual 6 Safety Instruc tions (cont' d.) Repairs, service work Refer to "Safety Conc ept" booklet ▶ Only original Leica M icrosystems spare ρ parts may be used. Before opening the instruments, switch off ρ the power and unplug the pow er cable.
Leica M-series User Manual 7 Safety Instruc tions (cont' d.) • Direct contact with eyepieces is a poten - tial transmission method for bacterial and viral infections of the eye. The risk can be kept to a minimum by using personal eyepieces for each individual or detachable eyecups.
Leica M-series User Manual 8 Safety Instruc tions (cont' d.) Supply unit Always unplug the supply unit from the power supply: When installing and disassembling the ρ lamp housing Before opening .
Leica M-series User Manual 9 Con tents General Instructions 2 Impor tant Safety Notes 3 Symbols Used 4 Safety Instructions 5 The Leica M Series for F luorescenc e Congra tulations! 1 3 A Step T owards.
Leica M-series User Manual 10 Quick Start Guid e The F astest Route to Success 6 4 Overview of an M S eries Microscope 6 5 The Corr ect Interpupillar y Distance 6 6 Using the Eyepiec es 6 7 F ocusing .
Leica M-series User Manual 11 Sy stem Illuminatio n Leica LED5000 MCI™ 10 6 Leica LED5000 RL 10 8 Leica EL6000 – About the Instrument 10 9 Leica EL 6000 – Operation 11 1 Leica EL 6000 – T roub.
Leica M-series User Manual 12 T he L eica M S eries f or F luoresc ence.
Leica M-series User Manual 13 Congr atula tions! Congra tulations on obtaining your new Leica M series stereomicroscope . W e are convinced it will exceed your expectations, as never before have we applied our decades of experience in the areas of optics, mechanical engineering and ergonomics in such an uncompr omising manner .
Leica M-series User Manual 14 A Step T owards Infinity Ever since their introduction by Horatio S. Greenough, stereomicroscopes have worked according to the optical principles based primarily on Ernst Abbe's research.
Leica M-series User Manual 15 The Electronics: Comf ort, Con v enience and Safety for Y our Experiments Never before have electronics been used as extensively in a L eica series as in the new M series lineup. Optics carrier , stand, base and illuminator are all connected using electrical contacts—which provides a number of advan - tages.
Leica M-series User Manual 16 Have a special r equest? Let us know! Leica Microsystems enjo ys an exceptional repu - tation when it comes to devising customer- specific solutions. If you have a special request that cannot be met with standard parts, c ontact your Leica consultant .
Leica M-series User Manual 17 Leica engineers were careful to ensure that the new Leica M series—like its predecessors— remains compatible with existing series.
Leica M-series User Manual 18 On W e Go If your new Leica microscope has already been assembled and commissioned by your Leica consultant, click her e to skip through the instal - lation instructions and go directly to the Quick Start Guide on Page 63 .
Leica M-series User Manual 19 Assembly.
Leica M-series User Manual 20 The first step is to c onnect the focusing column of the M series to the corresponding base . T ools used Hex socket screw driver , 3 mm ρ Assembling the column adapter 1. Securely install the column adapter on the column using the four included screw s.
Leica M-series User Manual 21 Assembling the F oc using Column with an Incident-ligh t Base When using an incident-light base, the focus - ing column and motorized focus are installed directly on the base; no extension plate is required . T ools used Hex socket screw driver , 3 mm ρ Assembly 1.
Leica M-series User Manual 22 Depending on the work situation, it is useful to restrict the maximum trav el path of the stereomicroscope. This prevents injury during handling of the sample caused by fingers or hands becoming trapped or the specimen touching the objective or even being damaged by it.
Leica M-series User Manual 23 T ools used Hex socket screw driver , 4 mm ρ Assembling the optics carrier 1. Place the optics carrier on the focusing column so that the screw fits into the thread provided and the lug fits int o the groov e. 2. Pr ess the optics carrier backwards to the focusing column and scr ew it in place using your other hand.
Leica M-series User Manual 24 T ube All intermediate tubes that fit between the optics carrier and the binocular tube are fitted in the same manner . T ools used No tools requir ed. ρ Prepara tions 1. Unscrew the positioning screw and r emove the protective co ver .
Leica M-series User Manual 25 Ey epieces T ools used No tools requir ed. ρ Magnification range Y ou can ex tend the overall mag nification range using available 10×, 16×, 25× and 40× wide - field eyepieces for persons w earing glasses. Prepara tion 1.
Leica M-series User Manual 26 Objective T ools used No tools requir ed. ρ Prepara tion 1. Remove the protective cap on the optics carrier by turning it. Attaching the objective • Hold the objective firmly during assem - bly and disassembly so that it does not fall onto the stage plate.
Leica M-series User Manual 27 UV Prot ection S creen: Assembly T ools used Allen key ρ Intended use The UV protection screen in front of the speci - men plane prevents the user from looking directly into the UV rays . Safety Notes • UV radiation can damage the eyes.
Leica M-series User Manual 28 Prepara tions • Hold the objectives firmly during assembly and disassembly so that they do not fall onto the stage plate . Move the drive housing all the way upwards ρ and remove the optics carrier , if the carrier has already been installed.
Leica M-series User Manual 29 Objective Nosepiece – A djusting Parfocality The following procedure only has to be carried out once. Afterwards, both objectives are par fo - cal, meaning that the specimen remains in focus when the objective nosepiece is rotated .
Leica M-series User Manual 30 T ransmitted-light Base TL ST Unpacking the base The base is deliver ed with the adapter plat e installed. Make sure the instruments are unpacked on a flat , sufficiently dimensioned, and non-slip sur face. Focusing driv e and column 1.
Leica M-series User Manual 31 FluoC ombi III™ – Preparations Before assembling the FluoCombi™, you have to disconnect all instruments from the pow er supply . F ailure to obser ve these instructions can result in damage to the micro - scope and the connected instruments.
Leica M-series User Manual 32 FluoC ombi III™ – Assembly 1. Guide the FluoC ombi III™ in from below , while holding it flat. Ensure that it is aligned with the holder on the focusing column at the left and right. 2. Hold the FluoC ombi III™ securely and screw it to the focusing column using the provided scr ew .
Leica M-series User Manual 33 Assembling the optics carrier 1. Unscrew the three screws and the adapter ring on the optics carrier . 2. Attach the optics carrier to the FluoCombi III and screw it into plac e. 3. Open the iris diaphragm complet ely . 4.
Leica M-series User Manual 34 FluoC ombi III™ – Adjusting the Parfocality Setting up the objective nosepiece The following configuration only has to be carried out once. It ensures that the par focality remains intact, and thus the speci - men does not need to be brought into focus again.
Leica M-series User Manual 35 FluoC ombi III™ – Adjusting the Parfocality (cont'd .) 9. By turning the objective ring, focus on the specimen un til the specimen appears abso - lutely sharp . 10. Enlarge the view just enough for the cen ter of the specimen to be visible in the field of view .
Leica M-series User Manual 36 4. Cent er the objec tive over the specimen by loosening or tightening the screw on one side of the objective holder while turning the screw on the other side in the opposite direction. FluoC ombi III™ – Centering the Objectives The following configuration only has to be carried out once.
Leica M-series User Manual 37 FluoC ombi III™ – Filter and UV Pr otection S creen Inserting the dichroic mirror 1. Pull the dummy filter carrier out of the holder . 2. Insert the corresponding dichroic mirror . Assembling the UV protection screen 1.
Leica M-series User Manual 38 Removing the transport anchors • Before you can use the transmitted- light base for the first time, it is abso - lutely necessar y to remove the two transpor t anchors.
Leica M-series User Manual 39 T ransmitted-light Base TL BFDF Standard delivery The base is deliver ed with the adapter plat e installed. The selected stage (Leica IsoPro™ mechanical stage or Leica standard stage 10 447 269), and the focusing drive will have to be mounted later .
Leica M-series User Manual 40 The base is deliver ed with the adapter plat e installed. The selected stage (Leica IsoPro™ mechanical stage or Leica standard stage 10 447 269), and the focusing drive will have to be mounted later . Ensure that the instruments are unpacked on a level, adequa tely sized, and nonskid underlay .
Leica M-series User Manual 41 IsoPro™ Manual Mechanical Stage: Assembly Before the Leica IsoP ro™ mechanical stage is mounted to the base, the axis containing the control buttons is attached either on the lef t or the right side of the mechanical stage.
Leica M-series User Manual 42 Leica IsoPro™ Manual Mechanical Stage: Assembly (con t' d.) 1. T ake the glass pla te from the mechanical stage and turn it around. 2. Change the gear rod from the left to the right-hand side. Control assembly 1. T ake the glass pla te from the mechanical stage and turn it around.
Leica M-series User Manual 43 Leica IsoPro™ Manual Mechanical Stage: Assembly (con t' d.) 3. Attach the mechanical stage evenly to the three threaded holes . 4. Now move the mechanical stage as far as it will go in the direction of the column. 5.
Leica M-series User Manual 44 Basics The transmitted light bases of the Leica TL series ( TL BFDF , TL RC™, TL RCI™) are supplied with an installed ex tension plate. The selected stage (Leica IsoPro™ mechanical stage or standard stage 10 447 269) and the focusing drive will have to be moun ted later .
Leica M-series User Manual 45 3. Remove the two M3 screws and the holder from the mechanical stage. 4. Remove the two M4 screws and the sleeve from the mechanical stage. 5. Remove the four shock-absorbing cartons from the mechanical stage. After remo val, keep all the transport anchors in the plastic bag provided for future transport.
Leica M-series User Manual 46 Leica IsoPro™ Mot orized Mechanical Stage: Assembly (cont' d.) The bases The TL BFDF , TL RC™ and RCI™ transmitted-light bases can be equipped with three different stages: Standard stage, manual and automated IsoPro™ mechanical stage .
Leica M-series User Manual 47 Leica IsoPro™ Mot orized Mechanical Stage: Assembly (cont' d.) Mechanical stage for X- Y stage control module 1. Plug the CTL2 plug of the mechanical stage motor into one of the three available C TL2 inter faces. 2.
Leica M-series User Manual 48 The new Leica M-series features extensive encoding with which various microscope data and settings can be read out, transferred to the PC and reproduc ed later .
Leica M-series User Manual 49 The integrated cable duc t in the column enables a neat cable layout around the microscope. F or example, the USB or FireW ire cables of the camera can be stow ed in the cable duct. Feeding the cables 1. Unscrew the three screws on the cable duct.
Leica M-series User Manual 50 Cables: Diagram TL RCI™ DCI Controlbo x PSC SmartT ouch PC USB Cross Stage Smart Move Pow er/USB LED5000 RL LED5000 MCI™ Footswit ch(es) Please use the Leic a A pplication Suite to configur e the functions of the system.
Leica M-series User Manual 51 The L eica LED5000 MCI™ (f or "Multi C ontrast Illumination") is installed using t wo screws. For optimum accessibility, the optics carrier should be remov ed during assembly (see Page 23.) Constrain ts The Leica LED5000 MCI™ cannot be used together with the objective nosepiece.
Leica M-series User Manual 52 Under certain cir cumstances, the light source must not be moved along with the optics carrier . A typical example is multifocus images in which the Z-stack changes while the angle of incidence of the light must remain the same.
Leica M-series User Manual 53 The ring illuminator , the Leica LED5000 RL ("Ring Light"), is installed on the objective using a single scr ew . It has been optimized for a work - ing distance between 60 and 70 mm. Constrain ts The Leica LED5000 RL can be used in conjunc - tion with the planapochromat 1× and planapo - chromat 0.
Leica M-series User Manual 54 • The manufacturer assumes no respon - sibility or liabilit y for any use outside of the intended use or use outside of the specifica - tions fr om Leica M icrosystems Wetzlar GmbH or any risks resulting fr om such use.
Leica M-series User Manual 55 Leica EL6000 – Safety Notes (cont' d.) • The Leica EL6000 compac t light source generates high-energy light with invisi - ble componen ts.
Leica M-series User Manual 56 Leica EL6000 – Safety Notes (cont' d.) Electrical safety F or indoor use only . Supply voltage 100–240 V~ (±10%) Fr equency 50–60 Hz Po wer consumption Max.
Leica M-series User Manual 57 Setting up the instrument • Set up the L eica EL6000 so that the fron t is readily accessible and visible . The ventilation slots on the sides and back ρ panel of the device must not be cov ered. Maintain a free room of at least 150 mm on ρ the rear panel of the instrument.
Leica M-series User Manual 58 Leica EL6000 – Prepar ation (c ont' d.) 4. Carefully lay the Leica EL6000 on its side so that the opening faces you . This makes it easier to insert the lamp. 5. Pull the pressure bolt back towards the front plat e using the lever .
Leica M-series User Manual 59 Leica EL6000 – Connection to the Fluor escenc e Lamp Housing • Always connec t the light guide to the lamp housing of the stereomicroscope in order to prevent danger to the user from the high-energy light. 1. Unscrew the positioning screw and r emove the protective cover of the fluorescence lamp housing.
Leica M-series User Manual 60 Leica EL6000 – Prepar ation (c ont' d.) 7. When the lamp makes full contact with the lamp mount, release the lev er . 8. Connect the plug of the lamp with the coupling in the instrument. Ensure that no wires are touching the reflector of the lamp.
Leica M-series User Manual 61 Leica EL6000 – Replacing the Lamp The procedure for replacing the lamp does not differ from the installation procedure outlined on Page 57, ex cept for the fac t that the existing lamp has to be remov ed first.
Leica M-series User Manual 62 Leica EL6000 – Connection to the Fluor escenc e Lamp Housing (cont' d.) 6. Insert the long end of the light guide into the light output of the Leica EL6000.
Leica M-series User Manual 63 Q uick Star t Guide.
Leica M-series User Manual 64 The F astest Route t o Success Y our Leica stereomicrosc ope has been delivered in completely assembled condition by your Leica par tner , and naturally you want to get right to work. Ther efore , your next step should be to study the Q uick Star t Guide, which outlines the most important steps at a glance.
Leica M-series User Manual 65 Overview of an M Series M icroscope 1 Eyepieces for spectacle wearers with dioptric correction and eyecups 2 Trinocular tube 3 Fluorescence housing 4 Filter Changer 5 Dis.
Leica M-series User Manual 66 The C orrect Interpupillary D istance The interpupillary distance is correctly set if you see a single circular image field when look ing at a specimen. If y ou ar e still a novice microscope user , you may need a shor t time to become accustomed to this.
Leica M-series User Manual 67 If you wear glasses: 1. Hold the eyepiece firmly and rotate the eyecups clockwise towards the rear , as otherwise the viewing distance is too great. 2. If an eyepiece is equipped with the inte - grated dioptric c orrection, turn the value to the "0" mark.
Leica M-series User Manual 68 F oc using F ocusing raises or lowers the stereomicroscope using the focusing drive. The specimen detail is brought into sharp focus as soon as it is in the focal point of the objective. The focusing drive can be operated either ρ left- or right-handed.
Leica M-series User Manual 69 Adjusting the Resistanc e of the F oc us Drive Adjusting the resistance Is the focus movement too loose or too tight? Does the outfit tend to slide downwards? The resistance can be adjusted individually depend - ing on the equipment weight and personal prefer ences as follow s: 1.
Leica M-series User Manual 70 Changing Magnification (Zoom) All M series microscopes have an integrated zoom. The name indicates the zoom range cover ed: Leica M165 C = 16.5:1 ρ Leica M205 C = 20.5:1 ρ The rotar y knob for the zoom can be used either left or right-handed.
Leica M-series User Manual 71 The zoom butt on can optionally be operat ed either with or without ratchet steps. Continu - ous z oom is possible when the ratchet steps are disabled, which many users find convenient. On the other hand, when the ratchet steps are enabled, photographs , measurement results etc.
Leica M-series User Manual 72 Parfocality: More Comf or t and Con venience for Y our W ork All Leica stereomicroscopes are parfocally matched, meaning that you can view a focused specimen from the lowest to the highest magnification without having to r efocus.
Leica M-series User Manual 73 The iris diaphragm in the optics carrier of your M series microscope fulfills the same purpose as the iris diaphragm in a camera: it regulates the av ailable light, which changes the depth of field.
Leica M-series User Manual 74 E y epieces.
Leica M-series User Manual 75 Magnification F ac tors of the Ey epieces An eyepiece not only makes it possible to look passively into the micro - scope, but also has a critical effect on the maximum magnification. The magnification factor is between 10x and 40x.
Leica M-series User Manual 76 Health Notes Pot ential sources of infection • Direct contact with eyepieces is a potential transmission method for bacterial and viral infections of the ey e. The risk can be kept to a minimum by using individual eyepieces or detachable eyecups.
Leica M-series User Manual 77 All Leica eyepiec es are also available with built- in dioptric correction, allowing the microscope to be used without glasses even by those with vision problems. The correction comprises ±5 diopter settings. Using the Dioptric Corr ection 1.
Leica M-series User Manual 78 Adjusting 1. Set the dioptric c orrection for both eyepieces to "0" . 2. Select the lowest magnification and focus on a flat specimen. 3. Select the highest magnification and read - just the sharpness. 4. Select the lowest magnification again, but do not look into the eyepiec es.
Leica M-series User Manual 79 Graticules 2. Clamp the graticule on the insert, applying moderate pressure. Ensure that the gr ati - cule fits tightly .
Leica M-series User Manual 80 Photogr aph y & V ideo.
Leica M-series User Manual 81 F or most microscope users, digital documen - tation has become an invaluable par t of their work. Research results can be presented in an attractive manner; measur ement.
Leica M-series User Manual 82 Photo T ubes and C-mounts Application All Leica DFC cameras are equipped with a standardized C-mount inter face. In turn, the C-mount adapter for the respective trinocular tube is connected t o this int er face.
Leica M-series User Manual 83 T rinoc ular Video/Phototube 50% Use With its third beam path, the trinocular video/ phototube 50% enables you to simultaneously view and photograph a specimen. The av ailable light is divided as follows: 50% is available for the two ey epieces.
Leica M-series User Manual 84 Use With its third beam path, the trinocular video/ phototube 100% enables you to either view or photograph a specimen. This means that 100% of the light is available to one or the other beam path. The other beam path remains opaque or black.
Leica M-series User Manual 85 Microsc ope Carrier.
Leica M-series User Manual 86 Use The objec tive nosepiece enables you to switch between two objectives using just one hand movement , f or example bet ween a 1× and a 2× planapochromat.
Leica M-series User Manual 87 Objec tiv es and Optic al A c c essories.
Leica M-series User Manual 88 The Differ ent T ypes of Objec tives T o meet the various requir ements regarding imaging properties, there is a choice of high- quality interchangeable planachromatic and planapochromatic objectives and also lower- priced inter changeable achromatic objectives.
Leica M-series User Manual 89 Bases.
Leica M-series User Manual 90 Leica TL ST T ransmitted-light Base: C ontrols 1 Adapter plate for easy assembly of focusing drives 2 Removable glass plat e 3 Controller for light int ensity 4 Adjustmen.
Leica M-series User Manual 91 Leica TL ST T ransmitted-light Base: Operation Light intensity contr ol The left control adjusts the intensity of the 12 V/20 W halogen illumination. 1. Switch on the illumination of the base at the power switch. 2. F ocus on the specimen.
Leica M-series User Manual 92 Changing the halogen lamp Before you change the lamp, it is abso - lutely necessary to unplug the power plug from the base to prev ent the risk of electric shock! The halogen lamp becomes ver y hot during operation.
Leica M-series User Manual 93 Leica TL BFDF T ransmitted-light Base: C ontrols 1 Adapter plate for easy assembly of focusing drives 2 Standard stage 10 447 269 3 Button to toggle between bright field.
Leica M-series User Manual 94 Light intensity contr ol • Observe the user manual—in par ticu - lar , all safety regulations—from the manufacturers of the light guide and cold light source . Switch on the cold light source ac cording to ρ the manufac turer's user manual and adjust the brightness.
Leica M-series User Manual 95 Leica TL RC™ / TL RCI™: Con trols V ertical column - transmitted-light base adapter plate 1 Heat sink of the integrated halogen illumina - tion (only TL RCI™) 2 Ext.
Leica M-series User Manual 96 Leica TL RCI™: The Deflection Mirror Inv erted world? Depending on the properties of the specimen (refractive index of the environment) and the perception of the viewer , it is possible that the switches described below for positive and inverted relief contrast are to be operated in r everse.
Leica M-series User Manual 97 Leica TL RCI™: Color Intensity and T emperature 1 Potentiometer for controlling the CCIC™ (Constant Color Intensity Control) illumina - tion intensity 2 Potentiometer.
Leica M-series User Manual 98 Setting the relief contrast The two switches on the left side of the trans - mitted-light base TL RC™/TL RCI™ activate two built-in flaps. The outer switch (1) controls the inverted relief contrast, and the inner switch (2) adjusts the positive relief c ontrast.
Leica M-series User Manual 99 Leica TL RCI™: Methods in T ransmitted Light V ertical bright-field illumination Suitable for stained amplitude specimens with sufficient contrast. The light beams are deflected ver tically through the specimen. This results in an accurate bright field with maximum brightness.
Leica M-series User Manual 100 Leica TL RCI™: Relief Images Starting position 1. Push the deflection mirror all the way back towar ds the column. 2. T urn the deflec tion mirr or into the notch position at an angle of 45°. Positiv e relief contr ast Suitable for semitransparent and transparent specimens.
Leica M-series User Manual 101 Dynamic relief contrast Suitable for semitransparent and transparent specimens. By tilting the deflection mirror slightly , you can move the gaps over the entire field of view and quickly toggle bet ween positive and negative relief images.
Leica M-series User Manual 102 Filters f or Leica TL RC™ and TL RCI™ The transmitt ed-light bases TL RC™ and TL RCI™ can be equipped with up to three filters— available as accessories—simultaneously . By customer request, the filters are also available as one- off items.
Leica M-series User Manual 103 Leica IsoPro™ (Non-mot orized): C ontrols Operating the Leica IsoPro™ mechanical stage 1. T o move the stage in the X direction, rotate the outer knob. 2. T o move the stage in the Y direction, rotate the inner control ring .
Leica M-series User Manual 104 Leica IsoPro™ (Mot orized): C ontrols 1 Leica IsoPro ™ motorized mechanical stage 2 Housing with motorization 1 Sub-D interface for Leica SmartMove™ 2 3 CTL2 inter.
Leica M-series User Manual 105 S y st em Illumina tion.
Leica M-series User Manual 106 Use The L eica LED5000 MCI™ (f or "Multi C ontrast Illumination") is a universal high-output illu - minator . Three groups of 3 LEDs each can be arranged as desired around the specimen. The targeted contr ol of the LEDs enables various illumination scenarios to be implement ed.
Leica M-series User Manual 107 Using the ke yboard Use the ρ key to switch the illumination on or off . Use the "+" and "–" keys to adjust the ρ brightness in 10 increments . T ouch either of the two butt ons to adjust the intensity in small increments.
Leica M-series User Manual 108 Leica LED5000 RL Use The Leica LED5000 RL ("Ring Light") generates a ver y bright and homogenous incident light. It has a diameter of 80 mm and illuminates the specimen with 48 LEDs that can be switched on and off completely or in various combinations.
Leica M-series User Manual 109 The Leica EL6000 compac t light source is intended for biological routine and research work, including the examination of specimens from the human body for the purpose of gain - ing information.
Leica M-series User Manual 110 Leica EL6000 – About the Instrument (c ont' d.) Compatible ligh t guides The Leica EL6000 compac t light source is compatible with liquid light guides that are compatible with a "Storz long" light inlet and whose transmission cur ve fits the lamp used.
Leica M-series User Manual 111 Leica EL 6000 – Operation Controls Running-time meter The running-time meter measures the burn - ing time of the lamp, not the switch-on time of the Leica EL6000 compact light source. The running-time meter displays its current status in this format: hours – minutes .
Leica M-series User Manual 112 Leica EL 6000 – T roubleshooting F ault description Possible faults Remedy Po wer indicator is not illumina ted after being switched on, instrument shows no function N.
Leica M-series User Manual 113 Cleaning and Maintenanc e • Before carr ying out cleaning tasks, switch off the Leica EL6000 compact light source . Use only a dry, lint-free cloth for cleaning. When cleaning, ensure that no dust or other foreign bodies enter the device thr ough the ven tilation slots.
Leica M-series User Manual 114 F luoresc enc e.
Leica M-series User Manual 115 Filt er Changer The patented FL UOIII® filter system consists of a filter changer for barrier and excitation filters, a UV shutter that can be activated/deactivated, and a filter inser t for a filter holder with your choice of filter .
Leica M-series User Manual 116 FIM – Fluor escence In tensity Manager FIM consists of a diaphragm disk with pinhole diaphragms of varying transmission capaci - ties. The intensity of the excitation light can be reduced in five fixed steps. The light can be changed from 100% to 55%, 30% and 17% down to 10%.
Leica M-series User Manual 117 About Fluor escenc e Microscop y Functional principle Fluoresc ent substances light up when irradi - ated with shor t-wave excitation light, and fluo - rescence microscop y takes advantage of this property. Specimens without self-fluorescence are stained with a fluorescent substance.
Leica M-series User Manual 118 Rapid Filter Changers and F ilter T ypes Filter sets Excitation filter Barrier filter Designation GFP 425/60 nm (395–455 nm) 480 LP GFP1 GFP Plus 480/40 nm (460–50.
Leica M-series User Manual 119 Simple filter holders Simple filter holders Three simple filter holders are included with the L eica M165 FC and the L eica M205 F A. These filter holders have t wo empty openings for the observation beam paths. The third opening, to the illumination beam path, is closed.
Leica M-series User Manual 120 Equipping the F ilter Changer • When inser ting the filter sets, av oid touching the filters in order to avoid fingerprints.
Leica M-series User Manual 121 Obser vation without F luorescence W e recommend that you first familiarize yourself with the contr ols of your Leica fluorescence microscope in transmitted light without the presence of fluorescence illumina - tion.
Leica M-series User Manual 122 Double Iris Aperture The built-in double -iris diaphragm regulates the depth of field. Note that: The greater the depth of field, the darker ρ the image becomes. In the same way , the exposure time ρ becomes longer for photog raphs.
Leica M-series User Manual 123 Commissioning the F luorescence S yst em After switching on the high-pressure mercury burner , you must adjust the ar c lamp imme - diately (see User Manual M2-216-1). Do not switch on the supply unit until you feel confi - dent using the Leica ster eomicroscope .
Leica M-series User Manual 124 A cc essories.
Leica M-series User Manual 125 With its non-slip controllers and k nobs, the Leica Smar tMove control unit offers the right feel for handling motoriz ed Leica instruments . Controls The functions of the individual controls can be configured both directly on the L eica Smart - Move and using the L eica Application Suite.
Leica M-series User Manual 126 With the touchscreen of the Leica Smar tT ouch™, you can control your experiment procedur es with a few hand mov ements and never lose sight of all your important optical parameters.
Leica M-series User Manual 127 Leica PSC Con troller Use The Leica PSC controller gives you precision control of the Leica IsoPr o™ mot orized mechan - ical stage. Connection Connect the Leica PSC controller and the Leica IsoPro™ motorized mechanical stage to the DCI box.
Leica M-series User Manual 128 Dimensional D ra wings.
Leica M-series User Manual 129 Leica M165 FC Leica M165 FC with incident light base and new focusing c olumn (dimensions in mm) 385 154 340 390 440 95 154 116 431 547 292 217 385 154 340 390 440 95 15.
Leica M-series User Manual 130 Leica M165 FC (cont' d.) Leica M165 C with TL RCI™ transmitted light stand , Leica IsoPro™ manual mechanical stage and manual focusing drive 497 292 217 187 154 max. 370 X = +-75 mm 370 95 300 249 Y = +-50 mm 116 431 547 497 292 217 187 154 max.
Leica M-series User Manual 131 Leica M165 FC (cont' d.) Leica M165 C with TL RCI™ transmitted light stand and manual f ocus 340 430 85 154 356 154 292 217 106 431 537 340 430 85 154 356 154 292.
Leica M-series User Manual 132 Leica M205 F A Leica M205 F A with TL BFDF tr ansmitted light stand, Ergo Trinoc tube , DFC camera and motorized f ocusing drive 370 154 340 390 90 154 292 116 431 547 3.
Leica M-series User Manual 133 Leica M205 F A (cont' d.) Leica M205 F A with TL RCI™ tr ansmitted light stand, Ergo Trinoc tube , DFC camera and motorized f ocusing drive 385 154 340 390 440 95.
Leica M-series User Manual 134 Leica M205 F A (cont' d.) Dimensions of Leica M205 F A with TL RCI™ transmitt ed light stand, IsoPr o™ mechanical stage, Ergo T rinoc tube, DFC camera and motoriz ed focusing drive 497 187 154 max. 410 X = +-75 mm 370 95 300 249 Y = +-50 mm 292 116 431 547 497 187 154 max.
Leica M-series User Manual 135 Leica LED5000 MCI 132 275.3 157 83.5 112 45 112 90 45 36 22 132 275.3 157 83.5 112 45 112 90 45 36 22 132 275.3 157 83.5 112 45 112 90 45 36 22 Leica LED5000 MCI (dimens.
Leica M-series User Manual 136 Leica LED5000 RL 120 ø70 30 153 153 ø80.
Leica M-series User Manual 137 MA TS TL 190 140 220 4 11 134 98 170 10 447 164 10 447 275.
Leica M-series User Manual 138 Specific a tions.
Leica M-series User Manual 139 Leica M165 FC / Leica M205 F A Leica M165 FC Leica M205 F A Optical data Zoom 16.5 : 1 manual 20.5 : 1 automated with F usionOptics™ Data with standard optics (1×objec - tive/10× eyepiec es) – Zoom range – Resolution – W orking distance – Object field 7.
Leica M-series User Manual 140 Leica M165 FC / Leica M205 F A (cont’ d.) Leica M165 FC Leica M205 F A Optics carrier 100% apochromatic optical syst em CMO (Common Main Objective) lead-fr ee CMO (Com.
Leica M-series User Manual 141 Leica M165 FC / Leica M205 F A (cont’ d.) Leica M165 FC Leica M205 F A Additional objectives (non-parfocal) 1× planachromat, 0.8× planapochr omat, 0.63× planapochromat, 0.5× planapochr omat 1× planachromat, 0.8× planapochr omat, 0.
Leica M-series User Manual 142 Leica M165 FC / Leica M205 F A (cont’ d.) Leica M165 FC Leica M205 F A Incident light stand Large incident light base with black/white stage pla te and Antishock™ fe.
Leica M-series User Manual 143 Leica EL6000 – Specifications Dimensions ( W×H×D) Width×height×depth 130 mm × 210 mm × 290 mm Environmen tal conditions Permitt ed ambient temperatur e -20 t o .
Leica M-series User Manual 144 Leica EL6000 – Specifications (cont' d.) Light output Design T ype: Storz, long Special feature Self-lock ing if no light guide is plugged in Fa n Switch-on delay Approx.
Leica M-series User Manual 145 Leica TL ST T ransmitted-light Base Light source Halogen lamp 12V/20W Quick illuminant change Ye s Illuminated area 50 mm Po wer supply unit Input voltage 100–240 V~, frequenc y 50–60 Hz energy consumption 30 W max., ambient temperatur e 10 – 40 °C Connections Power plug , power switch W eight 7.
Leica M-series User Manual 146 Leica TL BFDF T ransmitted-light Base Light source External via cold light source Illuminated area 40 mm Connections Connection for cold light guide , active f=10mm, end tube f=13mm W eight 5.
Leica M-series User Manual 147 Leica TL RC™ / TL RCI™ Leica TL RC™ Leica TL RCI™ Light source External via cold light source Halogen lamp 12V/20W Quick illuminant change – Ye s Illuminated area 35 mm 35 mm Po wer supply unit – 100–240 V~, frequency 50–60 Hz energy consumption 30 W max.
Leica M-series User Manual 148 Leica IsoPro™ Mot orized XY Stage Compatibility Leica inciden t light base and TL bases ( TL BFDF , TL RC™, TL RCI™) Stage size (L × W × H) 335.5 mm × 370 mm × 41.5 mm Tr a v e l 152 mm × 102 mm (6" × 4") Speed X,Y 30 mm/s Motor resolution X,Y 0.
Leica M-series User Manual 149 Appendix.
Leica M-series User Manual 150 Calculating the T otal Magnific ation and F ield of View Diameter Paramet er MO Magnification of the objective ME Magnification of the eyepiece z Magnification of the changer position q T ube factor , e .g. 1.5× for coaxial incident light, 1.
Leica M-series User Manual 151 Care , Maintenance, C ontac t P ersons W e hope you enjoy using your high-perfor - mance microscope. Leica microscopes are renowned for their r obustness and long ser vice life.
Leica M-series User Manual 152 Care , Maintenance, C ontac t P ersons (cont' d.) Protection from dirt Dust and dir t will affect the qualit y of your results. Put a dust cover over the microscope when ρ it will not be used for a long time. Use dust caps to protect tube openings, ρ tubes without eyepieces , and eyepieces.
Ein wichtiger Punkt beim Kauf des Geräts Leica M205 FA (oder sogar vor seinem Kauf) ist das durchlesen seiner Bedienungsanleitung. Dies sollten wir wegen ein paar einfacher Gründe machen:
Wenn Sie Leica M205 FA noch nicht gekauft haben, ist jetzt ein guter Moment, um sich mit den grundliegenden Daten des Produkts bekannt zu machen. Schauen Sie zuerst die ersten Seiten der Anleitung durch, die Sie oben finden. Dort finden Sie die wichtigsten technischen Daten für Leica M205 FA - auf diese Weise prüfen Sie, ob das Gerät Ihren Wünschen entspricht. Wenn Sie tiefer in die Benutzeranleitung von Leica M205 FA reinschauen, lernen Sie alle zugänglichen Produktfunktionen kennen, sowie erhalten Informationen über die Nutzung. Die Informationen, die Sie über Leica M205 FA erhalten, werden Ihnen bestimmt bei der Kaufentscheidung helfen.
Wenn Sie aber schon Leica M205 FA besitzen, und noch keine Gelegenheit dazu hatten, die Bedienungsanleitung zu lesen, sollten Sie es aufgrund der oben beschriebenen Gründe machen. Sie erfahren dann, ob Sie die zugänglichen Funktionen richtig genutzt haben, aber auch, ob Sie keine Fehler begangen haben, die den Nutzungszeitraum von Leica M205 FA verkürzen könnten.
Jedoch ist die eine der wichtigsten Rollen, die eine Bedienungsanleitung für den Nutzer spielt, die Hilfe bei der Lösung von Problemen mit Leica M205 FA. Sie finden dort fast immer Troubleshooting, also die am häufigsten auftauchenden Störungen und Mängel bei Leica M205 FA gemeinsam mit Hinweisen bezüglich der Arten ihrer Lösung. Sogar wenn es Ihnen nicht gelingen sollte das Problem alleine zu bewältigen, die Anleitung zeigt Ihnen die weitere Vorgehensweise – den Kontakt zur Kundenberatung oder dem naheliegenden Service.
